Husseini, Abbas AliYazdani, Ali MohammadGhadiri, FatemehSisman, Alper2024-09-112024-09-1120241618-02401618-2863https://doi.org/10.1002/elsc.202300230https://hdl.handle.net/11363/7555We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 mu L sample volume, glass particle size below 10 mu m, concentration of 0.2 mu g, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.eninfo:eu-repo/semantics/openAccesscell lysisdropletmicro-glass particlesurface acoustic waveArticle24110.1002/elsc.202300230381879282-s2.0-85178412744WOS:001113996600001Q2