Husseini, Abbas AliRostamzadeh, Mehran2024-09-112024-09-1120222322-181Xhttps://doi.org/10.22099/mbrc.2022.44692.1780https://hdl.handle.net/11363/8488The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program. The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepatitis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan © 2022, Molecular Biology Research Communications.All Rights Reserved.eninfo:eu-repo/semantics/closedAccessAfghanistan; Genotype; Hepatitis delta; Phylogenetic analysisArticle11418318610.22099/mbrc.2022.44692.17802-s2.0-85146671460Q3